Excitation and Disruption of a Giant Molecular Cloud by the Supernova Remnant 3C391

Using the IRAM 30-m telescope, we observed the supernova remnant 3C 391 (G31.9+0.0) and its surroundings in the CO(2-1), HCO+(1-0), CS(2-1), CS(3-2), and CS(5-4) lines. The ambient molecular gas at the distance (9 kpc) of the remnant comprises a giant molecular cloud whose edge is closely parallel to a ridge of bright non-thermal radio continuum, which evidently delineates the blast-wave into the cloud. We found that in a small (0.6 pc) portion of the radio shell, the molecular line profiles consist of a narrow (2 km/s) component, plus a very wide (> 20 km/s) component. Both spectral components peak within 20" of a previously-detected OH 1720 MHz maser. We name this source 3C 391:BML (broad molecular line); it provides a new laboratory, similar to IC 443 but on a larger scale, to study shock interactions with dense molecular gas. The wide spectral component is relatively brighter in the higher-excitation lines. We interpret the wide spectral component as post-shock gas, either smoothly accelerated or partially dissociated and reformed behind the shock. The narrow component is either the pre-shock gas or cold gas reformed behind a fully dissociative shock. Using the 3 observed CS lines, we measured the temperature, CS column density, and H2 volume density in a dense clump in the parent molecular cloud as well as the wide-line and narrow-line portions of the shocked clump. The physical conditions of the narrow-line gas are comparable to the highest-density clumps in the giant molecular cloud, while the wide-line gas is both warmer and denser. The mass of compressed gas in 3C 391:BML is high enough that its self-gravity is significant, and eventually it could form one or several stars

Cation-selective Mutations in the M2 Domain of the Inhibitory Glycine Receptor Channel Reveal Determinants of Ion-Charge Selectivity

Ligand-gated ion channel receptors mediate neuronal inhibition or excitation depending on their ion charge selectivity. An investigation into the determinants of ion charge selectivity of the anion-selective α1 homomeric glycine receptor (α1 glycine receptor [GlyR]) was undertaken using point mutations to residues lining the extra- and intracellular ends of the ion channel. Five mutant GlyRs were studied. A single substitution at the intracellular mouth of the channel (A-1′E GlyR) was sufficient to convert the channels to select cations over anions with PCl/PNa = 0.34. This result delimits the selectivity filter and provides evidence that electrostatic interactions between permeating ions and pore residues are a critical factor in ion charge selectivity. The P-2′Δ mutant GlyR retained its anion selectivity (PCl/PNa = 3.81), but it was much reduced compared with the wild-type (WT) GlyR (PCl/PNa = 27.9). When the A-1′E and the P-2′Δ mutations were combined (selectivity double mutant [SDM] GlyR), the relative cation permeability was enhanced (PCl/PNa = 0.13). The SDM GlyR was also Ca2+ permeable (PCa/PNa = 0.29). Neutralizing the extracellular mouth of the SDM GlyR ion channel (SDM+R19′A GlyR) produced a more Ca2+-permeable channel (PCa/PNa = 0.73), without drastically altering monovalent charge selectivity (PCl/PNa = 0.23). The SDM+R19′E GlyR, which introduces a negatively charged ring at the extracellular mouth of the channel, further enhanced Ca2+ permeability (PCa/PNa = 0.92), with little effect on monovalent selectivity (PCl/PNa = 0.19). Estimates of the minimum pore diameter of the A-1′E, SDM, SDM+R19′A, and SDM+R19′E GlyRs revealed that these pores are larger than the α1 GlyR, with the SDM-based GlyRs being comparable in diameter to the cation-selective nicotinic acetylcholine receptors. This result provides evidence that the diameter of the ion channel is also an important factor in ion charge selectivity

A Single P-loop Glutamate Point Mutation to either Lysine or Arginine Switches the Cation–Anion Selectivity of the CNGA2 Channel

Cyclic nucleotide-gated (CNG) channels play a critical role in olfactory and visual transduction. Site-directed mutagenesis and inside-out patch-clamp recordings were used to investigate ion permeation and selectivity in two mutant homomeric rat olfactory CNGA2 channels expressed in HEK293 cells. A single point mutation of the negatively charged pore loop (P-loop) glutamate (E342) to either a positively charged lysine or arginine resulted in functional channels, which consistently responded to cGMP, although the currents were generally extremely small. The concentration–response curve of the lysine mutant channel was very similar to that of wild-type (WT) channels, suggesting no major structural alteration to the mutant channels. Reversal potential measurements, during cytoplasmic NaCl dilutions, showed that the lysine and the arginine mutations switched the selectivity of the channel from cations (PCl/PNa = 0.07 [WT]) to anions (PCl/PNa = 14 [Lys] or 10 [Arg]). Relative anion permeability sequences for the two mutant channels, measured with bi-ionic substitutions, were NO3− > I− > Br− > Cl− > F− > acetate−, the same as those obtained for anion-selective GABA and glycine channels. The mutant channels also seem to have an extremely small single-channel conductance, measured using noise analysis of about 1–2 pS, compared to a WT value of about 29 pS. The results showed that it is predominantly the charge of the E342 residue in the P-loop, rather than the pore helix dipoles, which controls the cation–anion selectivity of this channel. However, the outward rectification displayed by both mutant channels in symmetrical NaCl solutions suggests that the negative ends of the pore helix dipoles may play a role in reducing the outward movement of Cl− ions through these anion-selective channels. These results have potential implications for the determinants of anion–cation selectivity in the large family of P-loop–containing channels

Analytic Perturbation Theory: A New Approach to the Analytic Continuation of the Strong Coupling Constant αS\alpha_S into the Timelike Region

The renormalization group applied to perturbation theory is ordinarily used to define the running coupling constant in the spacelike region. However, to describe processes with timelike momenta transfers, it is important to have a self-consistent determination of the running coupling constant in the timelike region. The technique called analytic perturbation theory (APT) allows a consistent determination of this running coupling constant. The results are found to disagree significantly with those obtained in the standard perturbative approach. Comparison between the standard approach and APT is carried out to two loops, and threshold matching in APT is applied in the timelike region.Comment: 16 pages, REVTeX, 7 postscript figure

Post-Inflationary Reheating

We study a model for reheating that has been much investigated for parametric resonance, having a quartic interaction of the scalar inflaton with another scalar field. Attention is particularly on the quantum excitations of the inflaton field and the metric perturbation with a smooth transition from quantum to classical stochastic states, followed through from a specific inflation model to a state including a relativistic fluid. The scalar fields enter non-perturbatively but the metric enters perturbatively, and the validity of this latter is assessed. In this model our work seems to point the large scale curvature parameter changing.Comment: 25 pages, 6 figures. Coding error(misprint) corrected:figures and some conclusions change

Cosmic microwave background and parametric resonance in reheating

The variation of the perturbative 3-curvature parameter, \zeta, is investigated in the period of reheating after inflation. The two-field model used has the inflaton, with an extra scalar field coupled to it, and non-linear effects of both fields are included as well as a slow decay mechanism into the hydrodynamic fluid of the radiation era. Changes in \zeta occur and persist into the succeeding cosmic eras to influence the generation of the cosmic microwave background fluctuations.Comment: 21 pages, 6 figures.Corrects misprinted formula and 2 number

Effects of Policies Designed to Keep Firearms from High-Risk Individuals

This article summarizes and critiques available evidence from studies published between 1999 and August 2014 on the effects of policies designed to keep firearms from high-risk individuals in the United States. Some prohibitions for high-risk individuals (e.g., those under domestic violence restraining orders, violent misdemeanants) and procedures for checking for more types of prohibiting conditions are associated with lower rates of violence. Certain laws intended to prevent prohibited persons from accessing firearms -- rigorous permit-to-purchase, comprehensive background checks, strong regulation and oversight of gun dealers, and requiring gun owners to promptly report lost or stolen firearms -- are negatively associated with the diversion of guns to criminals. Future research is needed to examine whether these laws curtail nonlethal gun violence and whether the effects of expanding prohibiting conditions for firearm possession are modified by the presence of policies to prevent diversion

Vector Meson Photoproduction with an Effective Lagrangian in the Quark Model

A quark model approach to the photoproduction of vector mesons off nucleons is proposed. Its starting point is an effective Lagrangian of the interaction between the vector meson and the quarks inside the baryon, which generates the non-diffractive s- and u- channel resonance contributions. Additional t-channel $\pi^0$ and $\sigma$ exchanges are included for the $\omega$ and $\rho^0$ production respectively to account for the large diffractive behavior in the small $t$ region as suggested by Friman and Soyeur. The numerical results are presented for the $\omega$ and $\rho$ productions in four isospin channels with the same set of parameters, and they are in good agreement with the available data not only in $\omega$ and $\rho^0$ productions but also in the charged $\rho$ productions where the additional t-channel $\sigma$ exchange does not contribute so that it provides an important test to this approach. The investigation is also extended to the $\phi$ photoproduction, and the initial results show that the non-diffractive behavior of the $\phi$ productions in the large $t$ region can be described by the s- and u- channel contributions with significantly smaller coupling constants, which is consistent with the findings in the similar studies in the QHD framework. The numerical investigation has also shown that polarization observables are essential for identifying so-called "missing resonances".Comment: 36 pages, 10 PS figures, extended version of nucl-th/9711061 and nucl-th/9803021, submitted to PR